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To obtain the necessary number of animals for the experiment, eight Wistar females were mated with eight Wistar males (1:1, individual cages) after checking the estrous cycle adapted from Marcondes and collaborators (2002)
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CO has well recognized protective effects in models associated with oxidative stress [32] and is a known inducer of GCL [33]
formerly called non-alcoholic fatty liver disease (NAFLD)), which can cause metabolic dysfunction-associated steatohepatitis (MASH) (formerly called nonalcoholic steatohepatitis (NASH))
At that concentration, 250 mcg equals 0.05 mL, or 5 units on a U-100 insulin syringe
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