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Rats were purchased from Karabuk University Experimental Medicine Application and Research Centre (DETUM), and the study was conducted in DETUM as specified in the ethics committee protocol
(Source 1) Suboptimal and deficient glutathione levels are associated with aging and an increased risk for chronic diseases, including: Mitochondrial dysfunction Neurodegenerative disease, including Alzheimers and Parkinsons disease Cancer Chronic liver disease Metabolic disease, including diabetes and hypertension Cardiovascular disease Pulmonary Infertility Autoimmune diseases, including lupus and multiple sclerosis Mental health disorders Cystic fibrosis (Source 1, 2) A viral illness, including SARS-CoV-2, the virus that causes Covid-19, can also cause glutathione deficiency
Quality of evidence: II-B, Strength of recommendation: 1 Panel level of agreement: In total agreement 96%, in partial agreement 4% The WHC have traditionally been used for diagnosing and grading the severity of clinically overt HE, but they are a subjective tool, with limited interobserver reliability, especially for a grade I classification