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For adipose, snap-frozen iWAT tissue was homogenized using a Dounce tissue homogenizer in 3 volume of 0.25 M sucrose, 2 mM EDTA, 0.1 M KPO 4 , pH 7 buffer containing 1:100 dilution of both phosphatase and protease inhibitors (Millipore, P8340 and P2850)
Multi-omic biomarkers associated with multiple sclerosis: from Mendelian randomization to drug prediction
Again these are reported mechanistic observations within experimental systems
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