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What is already known on this topic Glucagon-like peptide-1 (GLP-1) is involved in the development of impaired gallbladder motility Liraglutide, an important member of the GLP-1 receptor agonist class, has been reported to be associated with an increased risk of gallbladder or biliary diseases Glucose dependent insulinotropic polypeptide has also been reported to affect gallbladder motility What this study adds Dipeptidyl peptidase-4 (DPP-4) inhibitors were significantly associated with an increased risk of the composite of gallbladder or biliary diseases and cholecystitis DPP-4 inhibitors were not associated with increased risk of cholelithiasis and biliary diseases Risk of cholecystitis was increased with DPP-4 inhibitors compared with sodium-glucose cotransporter-2 inhibitors but not GLP-1 receptor agonists Ethics statements Ethical approval Not needed