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This is the step where the stability clock officially starts ticking, and a single misstep can compromise weeks, or even months, of valuable research
Uptake of CLF To measure CLF polarity, organoids were removed from the Matrigel by washing with PBS and incubated with culture medium supplemented with 10 g/ml CLF (Corning Inc.) and 1 g/ml Hoechst 33342 for 30 minutes at 37 C in a 5% CO 2 incubator
Glutamate, via conversion to -ketoglutarate, a key TCA cycle intermediate, serves an anaplerotic role in energy metabolism 73
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