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Average library size and concentration were determined using TapeStation and qPCR (NEBNext Library Quant kit, New England Biolabs), respectively, before pooling

Gene expression analysis by qPCR RNA was isolated from frozen tissues or fractionated adipocytes using TRIzol and the RNeasy Mini Kit (QIAGEN)

Researchers can find a COA published on their website

The American College of Obstetricians and Gynecologists (ACOG) and the Society for Maternal-Fetal Medicine (SMFM) continue to endorse acetaminophen as the safest option for fever reduction and mild pain management during pregnancy when used at the lowest effective dose for the shortest necessary duration