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Subcutaneous injection (most common): Injection sites: Abdomen (2 inches from the navel, any direction) Upper thighs (front or outer) Upper arms (outer aspect, if administered by an assistant) For localized treatment: near the injury site (within 23 inches) Procedure: Clean the injection site with an alcohol swab Let the alcohol dry completely Pinch the skin to create a fold of subcutaneous tissue Insert the needle at a 45-degree angle Inject slowly over 510 seconds Withdraw the needle smoothly Apply gentle pressure if needed (do not rub) Intramuscular injection (alternative): Injection sites: Deltoid (shoulder) Vastus lateralis (outer thigh) Gluteus medius (upper outer buttock) Procedure: Clean the site thoroughly Insert the needle at a 90-degree angle Aspirate slightly to confirm you are not in a blood vessel Inject slowly and steadily Withdraw the needle and apply pressure Dosing Frequency and Timing Frequency depends on research goals and injury characteristics

S.HoughtonO.ZengH.et al (1999)