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Complex was formed by adding 10 mM CaCl 2 , 10 mM MgCl 2 , 1 mM MnCl 2 , 50 mU/mL apyrase (Sigma-Aldrich, Darmstadt, Germany), 20 M GLP-2, 100 M TCEP (Sigma-Aldrich) and 10 g/mL Nb35 into the cell lysate and incubated at room temperature (RT) for 1.5 h

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(2) CCK-1R knockout rats exhibit a blunted response to peptone 190

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It is not a licensed medicine: Wegovy remains the sole semaglutide product approved for human weight-loss use in the UK as of 2026 [4][6], and all consumer-facing semaglutide supply is prescription-only under MHRA enforcement [3]

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