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Breifly, the reaction consists of 1 mM 1-chloro-2,4-dinitrobenzene (CDNB), 5 mM glutathione (GSH) in 50 potassium phosphate buffer and 500 l of crude enzyme in 1 ml total volume
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Furthermore, supplementation with extracellular GSH renders cancer cells resistant to drugs that block cystine uptake or its reduction into cysteine
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