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Membranes were subsequently blocked with 5% non-fat milk in TBST buffer for 2 h at room temperature and incubated overnight at 4 C with the appropriate primary antibodies as follows: anti-Bcl-2 antibody, anti-BAX antibody, anti-Cleaved Caspase 3 (Cl-Caspase 3) antibody, and anti-GAPDH antibody

Upon reaction with ROS or electrophiles, GSH becomes oxidized to GSSG, which can be reduced to GSH by the GSSG reductase (GR)

While both are highly effective, they have key differences in how they work, their effectiveness, and their side effect profiles

since Lean Pre-Workout contains caffeine, we decided to keep the creatine separate