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Lancet 383 , 896910 (2014)
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492 These findings suggest that hyperglycemia is only one of multiple factors contributing to neuropathy in T2DM, highlighting the need for a multifactorial approach to management
Samples were mixed with a nondenaturing PAGE loading buffer containing 5% (v/v) glycerol, bromophenol blue and Trisborate (TB) buffer (100 mM Tris and 100 mM boric acid) and run on a native PAGE gel (prerun at 200 V for 5 min) for 50 min (130 V, 4 C) in TB buffer
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