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The key consideration is ensuring sufficient subcutaneous fat at the chosen sitethe tissue should be soft and easily pinched to create a fold for injection
Recent studies of isolated L-cells (95) and a L-cell derived cell line, GLUTag cells, have shown stimulation of GLP-1 secretion by glucose acting to inhibit K+ channels as in islet beta cells, by activation of the SGLT-1 transporter leading to depolarization (91), or activation of sweet taste receptors (T1R2 / T1R3) which activate Gq and PLC
Although G q , G i , or G o protein complexes have been determined in these structures, the binding modes of ghrelin show minimal differences (Wang et al., 2021
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